Detection of homodimer formation of CD99 through extracelluar

mcd99 Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40. In the case of mCD99, two adjacent O- glycosylation sites, Thr-45 and Thr-50 (NMKPT 45 PKAPT 50 PKKPS) are relevant for PILR ␣ recognition (13). Sequence alnment of human

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